This list includes all players who have appeared in at least one regular season game for the Isotopes since 2003, a total of 358. Click on a player's name to view his career statistics.
| A (19) | 'TOPES | MLB SERVICE |
| Abercrombie, Reggie | 2007 | Marlins (06-07), Astros (08) |
| Abreu, Tony | 2009 | Dodgers (07, 09), Diamondbacks (10) |
| Aceves, Jonathon
| 2006 | - |
| Adkins, James
| 2010 | - |
| Aguila, Chris | 2004, 05-06 | Marlins (04-06), Mets (08) |
| Allen, Chad | 2003 | Twins (99-01), Indians (02), Marlins (03), Rangers (04-05) |
| Alvarado, Giancarlo | 2009 | - |
| Alvarez, Juan | 2003-04 | Angels (99-00), Rangers (02), Marlins (03) |
| Alvarez, Mario | 2011 | - |
| Anderson, Jimmy | 2006 | Pirates (99-02), Reds (03), Cubs (04), Red Sox (04) |
| Andino, Robert | 2006-08 | Marlins (05-08), Orioles (09-12) |
| Angle, Matt | 2012 | Orioles (11) |
| Antonini, Michael | 2012 | - |
| Ardoin, Danny | 2009 | Twins (00), Rangers (04), Rockies (05-06), Orioles (06), Dodgers (08) |
| Arias, Marlon | 2009 | - |
| Arlis, Patrick | 2007 | - |
| Arp, Ryan | 2009 | - |
| Ashby, Chris | 2003-07 | - |
| Ausmus, Brad | 2010 | Padres (93-96), Tigers (96, 99-00), Astros (97-98, 01-08), Dodgers (09-10) |
| Ayala, Luis | 2010 | Expos (03-04), Nationals (05, 07-08), Mets (08), Twins (09), Marlins (09), Yankees (11), Orioles (12) |
| B (31) |
| Baisley, Jeff | 2012 | Athletics (08) |
| Baker, Ryan | 2004 | - |
| Banks, Brian | 2004 | Brewers (96-99), Marlins (02-03)TD> |
| Bard, Josh | 2012 | Indians (02-05), Red Sox (06), Padres (06-08), Nationals (09), Mariners (10-11) |
| Barker, Kevin | 2003 | Brewers (99-00), Padres (02), Blue Jays (06), Reds (09) |
| Barnes, Larry | 2005 | Angels (01), Dodgers (03) |
| Barnwell, Chris | 2008 | Brewers (06) |
| Barone, Daniel | 2007-08 | Marlins (07) |
| Bastardo, Alberto | 2009-11 | - |
| Bauer, Peter | 2004-05 | - |
| Baugh, Kenny | 2007-08 | - |
| Beattie, Andrew | 2007-08 | - |
| Becker, Joe | 2011-12 | - |
| Bergman, Sean | 2003 | Tigers (93-95), Padres (96-97), Astros (98-99), Braves (99), Twins (00) |
| Bentz, Chad | 2005 | Expos (04), Marlins (05) |
| Blake, Casey | 2011 | Blue Jays (99), Twins (00-01), Orioles (01), Twins (02), Indians (03-08), Dodgers (08-11) |
| Blank, Matt | 2010 | Expos (00-01) |
| Blevins, Bobby | 2010 | - |
| Bochtler, Doug | 2003 | Padres (95-97), Tigers (98), Dodgers (99), Royals (00) |
| Borchard, Joe | 2007 | White Sox (02-05), Mariners (06), Marlins (06-07) |
| Borland, Toby | 2003-04 | Phillies (94-96, 98), Mets (97), Red Sox (97), Angels (01), Marlins (02-04) |
| Bonilla, Henry | 2009 | - |
| Bostick, Adam | 2006 | - |
| Bozied, Tagg | 2008 | - |
| Brinkley, Dante | 2008 | - |
| Broxton, Jonathan | 2011 | Dodgers (05-11), Royals (12) |
| Brower, Jim | 2011 | Indians (99-00), Reds (01-02), Expos (02), Giants (03-05), Braves (05), Orioles (06), Padres (06), Yankees (07) |
| Brown, Dee | 2009 | Royals (98-04), Athletics (07) |
| Bump, Nate | 2003-06 | Marlins (03-05) |
| Burnett, A.J. | 2004 | Marlins (99-05), Blue Jays (06-08), Yankees (09-11), Pirates (12) |
