In Chronological Order Of Debut
| Mike Costanzo | May 13, 2012 | Cincinnati Reds |
| Henry Rodriguez | September 2, 2012 | Cincinnati Reds |
| Didi Gregorius | September 5, 2012 | Cincinnati Reds |
| Pedro Villareal | September 5, 2012 | Cincinnati Reds |
| Tony Cingrani | September 9, 2012 | Cincinnati Reds |
| Justin Freeman | April 17, 2013 | Cincinnati Reds |
| Donald Lutz | April 29, 2013 | Cincinnati Reds |
| Curtis Partch | June 9, 2013 | Cincinnati Reds |
| Donnie Joseph | July 11, 2013 | Kansas City Royals |
| Billy Hamilton | September 3, 2013 | Cincinnati Reds |
| Tucker Barnhart | April 3, 2014 | Cincinnati Reds |
| Miguel Rojas | June 6, 2014 | Los Angeles Dodgers |
| Yohan Pino | June 19, 2014 | Kansas City Royals |
| Carlos Contreras | June 21, 2014 | Cincinnati Reds |
| Daniel Corcino | August 26, 2014 | Cincinnati Reds |
| Ryan Dennick | September 2, 2014 | Cincinnati Reds |
| Yorman Rodriguez | September 4, 2014 | Cincinnati Reds |
| Rey Navarro | April 24, 2015 | Baltimore Orioles |
| Michael Lorenzen | April 29, 2015 | Cincinnati Reds |
| Josh Ravin | June 2, 2015 | Los Angeles Dodgers |
| Jon Moscot | June 5, 2015 | Cincinnati Reds |
| Josh Smith | June 23, 2015 | Cincinnati Reds |
| Keyvius Sampson | July 30, 2015 | Cincinnati Reds |
| Kyle Waldrop | August 2, 2015 | Cincinnati Reds |
| Ryan LaMarre | September 22, 2015 | Cincinnati Reds |
| Robert Stephenson | April 7, 2016 | Cincinnati Reds |
| Drew Hayes | April 21, 2016 | Cincinnati Reds |
| Tim Adleman | May 1, 2016 | Cincinnati Reds |
| Layne Somsen | May 14, 2016 | Cincinnati Reds |
| Steve Selsky | May 20, 2016 | Cincinnati Reds |
| Daniel Wright | May 24, 2016 | Cincinnati Reds |
| Cody Reed | June 18, 2016 | Cincinnati Reds |
| Patrick Schuster | July 5, 2016 | Oakland Athletics |
| Tony Renda | August 2, 2016 | Cincinnati Reds |
| Wandy Peralta | September 4, 2016 | Cincinnati Reds |
| Barrett Astin | April 3, 2017 | Cincinnati Reds |
| Rookie Davis | April 6, 2017 | Cincinnati Reds |
| Amir Garrett | April 7, 2017 | Cincinnati Reds |
| Jesse Winker | April 14, 2017 | Cincinnati Reds |
| Sal Romano | April 16, 2017 | Cincinnati Reds |
| Phillip Ervin | April 22, 2017 | Cincinnati Reds |
| Ariel Hernandez | April 24, 2017 | Cincinnati Reds |
| Ben Lively | June 3, 2017 | Philadelphia Phillies |
| Luis Castillo | June 23, 2017 | Cincinnati Reds |
| Kevin Shackelford | June 29, 2017 | Cincinnati Reds |
| Jackson Stephens | July 1, 2017 | Cincinnati Reds |
| Alejandro Chacin | August 23, 2017 | Cincinnati Reds |
| Tyler Mahle | August 27, 2017 | Cincinnati Reds |
| Chad Wallach | August 27, 2017 | Cincinnati Reds |
| Zach Vincej | September 1, 2017 | Cincinnati Reds |
| Fabio Castillo | September 2, 2017 | Kansas City Royals |
| Deck McGuire | September 12, 2017 | Cincinnati Reds |
| Keury Mella | September 20, 2017 | Cincinnati Reds |
| Marcus Walden | April 1, 2018 | Boston Red Sox |
| Tanner Rainey | April 10, 2018 | Cincinnati Reds |
| Alex Blandino | April 11, 2018 | Cincinnati Reds |
| Zack Weiss | April 12, 2018 | Cincinnati Reds |
| Brandon Dixon | May 22, 2018 | Cincinnati Reds |
| Robert Stock | June 24, 2018 | San Diego Padres |
| Jesus Reyes | April 4, 2018 | Cincinnati Reds |
| Aristedes Aquino | August 19, 2018 | Cincinnati Reds |
| Blake Trahan | September 3, 2018 | Cincinnati Reds |
| Gabby Guerrero | September 4, 2018 | Cincinnati Reds |
| Joe Hudson | September 8, 2018 | Los Angeles Angels |
| Nick Senzel | May 3, 2019 | Cincinnati Reds |
| Josh VanMeter | May 5, 2019 | Cincinnati Reds |
| Shed Long | May 11, 2019 | Seattle Mariners |
| Luis Arraez | May 18, 2019 | Minnesota Twins |
| Devin Smeltzer | May 28, 2019 | Minnesota Twins |
| Sean Poppen | June 19, 2019 | Minnesota Twins |
| Jimmy Herget | July 7, 2019 | Cincinnati Reds |
| Cody Stashak | July 23, 2019 | Minnesota Twins |
| Brian O'Grady | August 8, 2019 | Cincinnati Reds |
| Randy Dobnak | August 9, 2019 | Minnesota Twins |
| Brusdar Graterol | September 1, 2019 | Minnesota Twins |
| Jaylin Davis | September 4, 2019 | San Francisco Giants |
| Seth Mejias-Brean | September 4, 2019 | San Diego Padres |
| Jorge Alcala | September 21, 2019 | Minnesota Twins |
| Aaron Whitefield | July 25, 2020 | Minnesota Twins |
| Chadwick Tromp | July 29, 2020 | San Francisco Giants |
| Lewin Diaz | August 15, 2020 | Miami Marlins |
| Ryan Jeffers | August 20, 2020 | Minnesota Twins |
| Brian Navarreto | August 23, 2020 | Miami Marlins |
| Robinson Leyer | September 1, 2020 | Boston Red Sox |
| Domingo Tapia | September 11, 2020 | Boston Red Sox |
| Travis Blankenhorn | September 15, 2020 | Minnesota Twins |
| Edwar Colina | September 25, 2020 | Minnesota Twins |
| Alex Kirilloff | September 30, 2020 | Minnesota Twins |
| Sam Clay | April 7, 2021 | Washington Nationals |
| Ben Rortvedt | April 30, 2021 | Minnesota Twins |
| Trevor Larnach | May 8, 2021 | Minnesota Twins |
| Bailey Ober | May 18, 2021 | Minnesota Twins |
| Vladimir Gutierrez | May 28, 2021 | Cincinnati Reds |
| Griffin Jax | June 8, 2021 | Minnesota Twins |
| Tony Santillan | June 13, 2021 | Cincinnati Reds |
| Charlie Barnes | July 17, 2021 | Minnesota Twins |
| Dusten Knight | August 4, 2021 | Baltimore Orioles |
| Sean Guenther | August 4, 2021 | Miami Marlins |
| Marcos Diplan | August 6, 2021 | Baltimore Orioles |
| Edward Cabrera | August 25, 2021 | Miami Marlins |
| Jose Siri | September 3, 2021 | Houston Astros |
| Jovani Moran | September 12, 2021 | Minnesota Twins |
| TJ Friedl | September 18, 2021 | Cincinnati Reds |
| Nick Fortes | September 18, 2021 | Miami Marlins |
| Jhoan Duran | April 8, 2022 | Minnesota Twins |
| Cole Sands | May 1, 2022 | Minnesota Twins |
| Jose Miranda | May 2, 2022 | Minnesota Twins |
| Royce Lewis | May 6, 2022 | Minnesota Twins |
| Mark Contreras | May 11, 2022 | Minnesota Twins |
| Jason Alexander | June 1, 2022 | Milwaukee Brewers |
| Chris Okey | June 10, 2022 | Cincinnati Reds |
| Aneurys Zabala | June 12, 2022 | Miami Marlins |
| Jerar Encarnacion | June 19, 2022 | Miami Marlins |
| Narciso Crook | June 30, 2022 | Chicago Cubs |
| Max Meyer | July 16, 2022 | Miami Marlins |
| Caleb Hamilton | July 17, 2022 | Minnesota Twins |
| J.J. Bleday | July 23, 2022 | Miami Marlins |
| Brennan Bernardino | July 31, 2022 | Seattle Mariners |
| Peyton Burdick | August 5, 2022 | Miami Marlins |
| A.J. Ladwig | August 14, 2022 | Miami Marlins |
| Andrew Nardi | August 16, 2022 | Miami Marlins |
| Bryan Hoeing | August 20, 2022 | Miami Marlins |
| Jeff Lindgren | April 3, 2023 | Miami Marlins |
| George Soriano | April 16, 2023 | Miami Marlins |
| Drew Maggi | April 26, 2023 | Pittsburgh Pirates |
| Eury Pérez | May 12, 2023 | Miami Marlins |
| Jordan Balazovic | June 18, 2023 | Minnesota Twins |
| Dane Myers | July 4, 2023 | Miami Marlins |
| Robert Garcia | July 14, 2023 | Miami Marlins |
| Kyle Nicolas | September 19, 2023 | Pittsburgh Pirates |
| Nasim Nuñez | March 30, 2024 | Washington Nationals |
| Tyler Jay | April 11, 2024 | New York Mets |
| Anthony Maldonado | April 26, 2024 | Miami Marlins |
Number Of Blue Wahoos To Play In The Majors: 133
First Call-Up
Mike Constanzo
Position: First Base
In Pensacola: 2012
MLB Debut: May 13, 2012
Debut Team: Cincinnati Reds
Most Recent
Anthony Maldonado
Position: Right-handed Pitcher
In Pensacola: 2021-22
MLB Debut: April 26, 2024
Debut Team: Miami Marlins